Phase Separator Columns are efficient to separate aqueous phase from chlorinated solvents under gravity. These ready-to-use cartridges are fitted with a proprietary hydrophobic frit and are a great alternative to liquid-liquid extraction, the most popular technique to do this separation. However, this last method is time consuming, requires the use of a glass funnel which needs to be washed between each separate extraction and is not suitable for multiple extractions. Phase Separator Columns solve these drawbacks and offer many advantages such as: Ease of use and Efficient and cost saving, Comply with “Green Chemistry” philosophy, and are Compatible with automated systems.


Typical Experimental Procedure Phase Separators

  1. Select the appropriate size of a Phase Separator Column to hold the entire sample volume (both aqueous and chlorinated phases).
  2. Connect the Phase Separator Column on a vacuum manifold. Ensure the collection vessel volume is sufficient enough to recuperate entirely the organic layer. (Note: Do not connect the manifold to a vacuum source.)
  3. Transfer the sample mixture to be separated on the top of the Phase Separator Column.
  4. After a few seconds (under gravity), the water immiscible chlorinated solvent will start to pass through the frit and is collected in the suitable vial already placed inside the manifold.
  5. The proprietary frit used in the Phase Separator Column allows the aqueous layer to be left on the column for at least 48 hours without passing through the frit.

phase sep

Important Advices

  • Process under gravity only - Do not apply vacuum or positive pressure. The Phase Separator Columns are designed to be used under gravity only. The use of vacuum or positive pressure source can yield to a lost in the separation efficiency.
  • Biphasic or two phase system required
  • The sample to be separated needs to contain water and a water immiscible solvent with greater density then water to form the lower layer. Most common solvents are dichloromethane, chloroform and chlorinated solvents. Furthermore, try to minimize the presence of water miscible solvent (i.e. methanol, ethanol or acetone) which can cause problem to obtain a real biphasic system and consequently, the phase separator may not work effectively.

*To obtain a most efficient compound partition between the aqueous and the organic layer, a liquid-liquid extraction can be done prior to use the phase separator column.