Polar Chromatographic Stationary Phases :
Part number , Name , Description , Loading
R35030B : SiliaBond Diol Scavenger and Chromatographic
Stationary Phase, 9 % carbon 1.1 mmol / g
R38030B : SiliaBond Cyano Chromatographic Stationary Phase,
7 % carbon
R52030B : SiliaBond Amine Base, Metal Scavenger, Chromatographic
Stationary Phase, and Ion Exchanger 1.6 mmol / g, 9 % carbon
R63030B : SiliaBond Cyclohexyldiol Other 10 % carbon,
1 mmol / g
Product Data Sheet :
Part Number: R35030B
Loading: 9 % carbon
1.1 mmol / g
Particle Size: 40 - 63 µm
Pore Size: 60
Specific surface area: 500 m²/g
Description: Scavenger and Chromatographic Stationary Phase
Technical Information
Synthesis: SiliaBond Diol may be used as boronic acid scavenger in the same way as PS-DEAM.
Separation
SiliaBond Diol may be used as polar sorbent in normal phase and aqueous size exclusion chromatography. Like bare silica, SiliaBond Diol has the ability to form hydrogen bonds and also the capacity to separate structural isomers. Since most of its surface is covered with organic functions, the SiliaBond Diol absorbs less water which leads to a more reproducible activity. It is also the sorbent of choice when working in normal phase in the presence of water. It has a different selectivity than bare silica gel and slight modifications in the composition of the solvent mixture may be necessary to obtain a similar retention. In SPE, it is used for the isolation of drugs and metabolites from physiological fluids.
Phenylboronic acid scavenging
with SiliaBond Diol
Equivalents - Time - Efficiency
2 equiv., 1 hour , 75%
4 equiv., 1 hour , 100%
Analyzed by GC-MS
Sample Procedures
Add 2-4 eq. to the crude reaction
mixture, stir for 1 h, then filter.
Solvent compatibility: All solvents, aqueous and organic
Prolonged storage: Keep dry
Product Data Sheet :
Part Number: R38030B
Loading: 7 % carbon
Particle Size: 40 - 63 µm
Pore Size: 60
Specific surface area: 500 m²/g
Description: Chromatographic Stationary Phase
Technical Information
SiliaBond Cyano (or Si-CN) can
be used both in normal and reversed-phase chromatography as its polarity marks
the separation between the polar and non polar phases. SiliaBond Cyano is
the least retentive of the polar sorbents in normal phase chromatography and
the least retentive of the non polar sorbents in reversed-phase chromatography.
This sorbent is particularly useful when dealing with extremes in the polarity
scale since it reduces the retention time and helps to improve peaks shape.
Solvent compatibility: All solvents, aqueous and organic
Prolonged storage: Keep dry
SiliaBond Amine
Product Data Sheet :
Part Number: R52030B
Loading: 1.6 mmol / g
9 % carbon
Particle Size: 40 - 63 µm
Pore Size: 60
Specific surface area: 500 m²/g
Description: Base, Metal Scavenger, Chromatographic Stationary Phase, and
Ion Exchanger
Technical Information
Synthesis
SiliaBond Amine (or Si-NH2) is an effective scavenger of acid chlorides1, sulfonyl chlorides, isocyanates and other electrophiles. PS-Trisamine has been compared to SiliaBond Amine for the removal of acid chlorides in the synthesis of 2,9-disubstituted guanines (scheme 1).1 The use of SiliaBond Amine to remove excess acid chloride gave higher yields (30-50%) higher) and better purity in 15 of the 16 reactions compared to PS-trisamine.
SiliaBond Amine eliminates tedious post reaction purification. It scavenges faster, is easier to add and filter, and can be used in any solvent.
Silica-supported amine has been shown to be an effective metal scavenger2 and catalyst for Knoevenagel reactions.3,4 It has also been used as support in solid-phase chemistry for chemical peptide synthesis followed by enzymatic hydrolysis5 and for Claisen rearrangement.6
Separation
SiliaBond Amine is also used in chromatography as a normal phase sorbent. It is used in the separation of peptides, drugs, and metabolites from physiological fluids. Also used for the separation of mono- and polysaccharides. It is more retentive towards basic compounds. When used at pH = 7.8, SiliaBond Amine is protonated and may be used as a weak anion exchanger (WAX).
This functionalized silica gel has been used for the separation of solid-phase extraction of sugars7, as well as for the separation of steroids8, cholesterol, and triglycerides.9 It has also been used in size exclusion chromatography of cationic polymers.10
Also available non-endcapped (R52130B) for additional retention of polar and cationic compounds.
Related Publications
1) Tetrahedron Lett., 41 (2000) 3573
2) Chem. Commun., (2000) 1145
3) Tetrahedron Lett., 29 (1998) 2261
4) J. Chem. Soc. Perkin Trans. I, (1989) 105
5) Tetrahedron Lett., 44 (2003) 6083
6) Molecular Diversity, 3 (1998) 161
7) J. Liq. Chromatogr., 14 (1991) 1185
8) J. Chromatogr., 392 (1987) 464
9) J. Chromatogr., 434 (1988) 395
10) J. Chromatogr., 389 (1987) 103
Sample Procedures : Add 2-4 eq. to the crude reaction mixture, stir for 1
h, then filter.
Solvent compatibility: All solvents, aqueous and organic
Prolonged storage: Keep cool
(<8°C) and dry
Product Data Sheet :
Part Number: R63030B
Loading: 10 % carbon
1 mmol / g
Particle Size: 40 - 63 µm
Pore Size: 60
Specific surface area: 500 m²/g
Description: Other
Technical Information: No
specific application available for this product.
Solvent compatibility: All solvents aqueous and organic
Prolonged storage: Keep dry