Non Polar phases

SiliaBond Silver Nitrate

Product Data Sheet:
Part Number: R23530B
Loading: 10 % wt / wt
Particle Size: 40 - 63 µm
Pore Size: 60
Specific surface area: 500 m²/g
Description: Chromatographic Stationary Phase

Technical Information

Chromatography with silver nitrate is a well known and established methodology for the separation of non polar compounds with similar polarities. Generally, with a mixture of unsaturated compounds such as alkenes, lipids, steroids, terpenes, etc, standard separation procedures lack in efficiency. However, by using SiliaBond Silver Nitrate for your column chromatography along with SiliaPlate Ag for your TLC, you will have the necessary tools to circumvent the problem. The theory behind this separation is based on the fact that silver ions can complex with unsaturated compounds (p bond). This kind of chromatography with silver nitrate impregnated silica gel is very easy to perform and is well documented1-4.

1) Tetrahedron, 57 (2001) 425

2) J. Chromatogr. A, 715 (1995) 372

3) J. Chromatogr., 38 (1968) 535

4) Microchem. J., 9 (1965) 227

Handling Procedures

Material is light sensitive. Exposure prior to use should be minimized. The material works best when eluted with hexane or heptane mixed with small quantities of polar solvents such as ethyl acetate or ethanol.

Sample separation:Cholesterol (Rf: 0.37) and Cholestanol (Rf: 0.37) eluted with hexane/EtOAc (3:1)

Storage: Keep dry and covered

Solvent compatibility: All organic solvents

Prolonged storage: Keep dry and covered (light sensitive)

SiliaBond C18 (23%)

Product Data Sheet:
Part Number: R30030B
Loading: 23 % carbon
Particle Size: 40 - 63 µm
Pore Size: 60
Description: Chromatographic Stationary Phase

Technical Information

SiliaBond C18 (or C18) is the traditional matrix for reversed-phase chromatography. The high loading provides the highest degree of hydrophobicity commercialy available. It is considered as the least selective phase since it interacts with a wide range of organic molecules. Also used in ion-pairing chromatography for the separation of biomolecules.

Solvent compatibility: All solvents, aqueous and organic

Prolonged storage: Keep dry

C18 not endcapped(NEW)

Product Data Sheet:
Part Number: R30130B
Loading: 17 % carbon
Particle Size: 40 - 63 µm
Pore Size: 60
Specific surface area: 500 m²/g
Description: water wettable C18

Technical Information
This C18 does not require preconditioning with organic solvents

Solvent compatibility:
all solvents aqueous and organic

SiliaBond C18 (17%)

Product Data Sheet :
Part Number: R30230B
Loading: 17 % carbon
Particle Size: 40 - 63 µm
Pore Size: 60
Specific surface area: 500 m²/g
Description: Chromatographic Stationary Phase

Technical Information
SiliaBond C18 (or C18) is the traditional matrix for reversed-phase chromatography. The high loading provides the highest degree of hydrophobicity commercialy available. It is considered as the least selective phase since it interacts with a wide range of organic molecules. Also used in ion-pairing chromatography for the separation of biomolecules.

Solvent compatibility: All solvents, aqueous and organic

Prolonged storage: Keep dry

SiliaBond C18 (11%)

Product Data Sheet:
Part Number: R30430B
Loading: 11 % carbon
Particle Size: 40 - 63 µm
Pore Size: 60
Specific surface area: 500 m²/g
Description: Chromatpgraphic Stationary Phase

Technical Information
SiliaBond C18 (or C18) is the traditional matrix for reversed-phase chromatography. The high loading provides the highest degree of hydrophobicity commercialy available. It is considered as the least selective phase since it interacts with a wide range of organic molecules. Also used in ion-pairing chromatography for the separation of biomolecules.

Solvent compatibility: All solvents, aqueous and organic

Prolonged storage: Keep dry

SiliaBond C8

Product Data Sheet:
Part Number: R31030B
Loading: 12 % carbon
Particle Size: 40 - 63 µm
Pore Size: 60
Specific surface area: 500 m²/g
Description: Chromatographic Stationary Phase

Technical Information

SiliaBond C8 (or C8) is a reversed-phase matrix with moderate degree of hydrophobicity that works well for separating a wide range of compounds. May be used as replacement of C18 when shorter retention times are desired or required.

Solvent compatibility: All solvents, aqueous and organic

Prolonged storage: Keep dry

SiliaBond C4

Product Data Sheet:
Part Number: R32030B
Loading: 8 % carbon
Particle Size: 40 - 63 µm
Pore Size: 60
Specific surface area: 500 m²/g
Description: Chromatographic Stationary Phase

Technical Information

SiliaBond C4 (or C4) provides less retention of non-polar compounds than C18 and C8 and is useful in ion-pairing chromatography. Used to separate large biomolecules. It works best for molecules with large hydrophilic region or in case wher the hydrophobic region is buried within the 3D structure.

Solvent compatibility: All solvents, aqueous and organic

Prolonged storage: Keep dry

SiliaBond C1

Product Data Sheet :
Part Number: R33030B
Loading: 5 % carbon
Particle Size: 40 - 63 µm
Pore Size: 60
Specific surface area: 500 m²/g
Description: Chromatographic Stationary Phase

Technical Information
SiliaBond C1 (or C1) has the lowest degree of hydrophobicity of all reversed stationary phases, useful in the separation of large bio-molecules that have extensive hydrophobic regions.

Solvent compatibility: All solvents, aqueous and organic

Prolonged storage: Keep dry

SiliaBond Phenyl

Product Data Sheet
Part Number: R34030B
Loading: 9 % carbon
Particle Size: 40 - 63 µm
Pore Size: 60
Specific surface area: 500 m²/g
Description: Chromatographic Stationary Phase

Technical Information

SiliaBond Phenyl (or Si-PHE) is a sorbent of medium polarity used in reversed-phase chromatography. It has similar retention to C8 but with a different selectivity especially for molecules containing aromatics and fatty acids.

Solvent compatibility: All solvents, aqueous and organic

Prolonged storage: Keep dry

SiliaBond C12

Product Data Sheet :
Part Number: R53030B
Loading: 16 % carbon
Particle Size: 40 - 63 µm
Pore Size: 60
Specific surface area: 500 m²/g
Description: Chromatographic Stationary Phase

Technical Information

SiliaBond C12 (or C12, Si-Adamantyl) has similar polarity to C18 with additional steric bulk to provide additional separation characteristics.

Solvent compatibility: All solvents, aqueous and organic

Prolonged storage: Keep dry

SiliaBond Cyclohexyl

Product Data Sheet:
Part Number: R61530B
Loading: 10 % carbon
Particle Size: 40 - 63 µm
Pore Size: 60
Specific surface area: 500 m²/g
Description: Chromatographic Stationary Phase

Technical Information

SiliaBond Cyclohexyl (or Si-CYH) is a sorbent of medium polarity used in reversed-phase chromatography. It has a different selectivity than the alkyl sorbents (C18, C8, C4...) and phenyl and may be used when these fail to provide the desired selectivity.

Solvent compatibility: All solvents, aqueous and organic

Prolonged storage: Keep dry